| Summary |
The purpose of this research is to use electroporation to determine which promoter will produce higher numbers of blue stained cells that express the LacZ gene within channel catfish G5F fibroblasts. The two promoters used in this study were the cytomegalovirus (CMV) promoter and the catfish P-actin promoter. Catfish G5F fibroblasts were cultured and underwent electroporation to be transfected with each promoter construct. A LacZ enzyme staining assay was performed to detect for the expression of the LacZ gene. Following the LacZ enzyme assay statistical comparisons were made between both promoters to determine statistical significance. The results from this experiment support the common choice of the CMV promoter as a popular promoter for gene expression. The catfish p-actin promoter did not perform at the same expression level as the CMV promoter within the channel catfish G5F fibroblasts. Based on the statistical results from a paired T-test the P value was less than 0.05 and provided statistical significance that the CMV promoter provided higher expression levels of the LacZ gene than the catfish P-actin promoter. These results confined the fact that the CMV promoter would be a more suitable promoter to use with channel cattish G5F llbroblasts for the expression of exogenous molecules, such as MHC class 11. |
| General note | Presented to the faculty of the Department of Biology. |
| General note | Advisor: Thomas J. McConnell |
| Dissertation note | M.S. East Carolina University 2008 |
| Bibliography note | Includes bibliographical references (leaves 77-86). |
| Genre/form | dissertations. |
| Genre/form | Academic theses. |
| Genre/form | Academic theses. |
| Genre/form | Thèses et écrits académiques. |
