| Variant title |
The cytoprotective properties of Prostaglandin E₂ against the toxic effects of Actinomycin C on embryonal neural retina cells. |
| Summary |
Prostaglandins (PGs) have been shown to cytoprotect various tissue types against the toxic effects of many chemicals. The mechanism of this protection is poorly understood, but the involvement of cAMP is often implied. There is however, only one previous study in regard to nervous tissue. The present study was designed to determine if PGE2 affords cytoprotection to embryonal neural retina cells (a more specialized nervous tissue) from the toxicity of actinomycin C (AMC) as determined by the Trypan Blue exclusion assay. Further, is this protection dose dependent, and what is the possible mechanism. The lowest concentration of PGE2 [2.0 x 10 M] had no effect on neural retina cells, but as the concentration increased [3.0 x 10 M and 5.0 x 10 M], PGE2 did afford protection against AMC in a dose dependent fashion. Neither theophylline nor phosphodiesterase demonstrated cytoprotective properties, suggesting that cAMP may not be involved in cytoprotection. These results suggested that PGE2 affords cytoprotection at elevated concentrations, and this effect was dose related. Evidence did not suggest the involvement of cAMP in this process. |
| General note | Submitted to the faculty of the Department of Biology. |
| General note | Advisor: Gerhard W. Kalmus |
| Dissertation note | M.S. East Carolina University 1990 |
| Bibliography note | Includes bibliographical references (leaves 44-47). |
| Genre/form | Academic theses. |
| Genre/form | Academic theses. |
| Genre/form | Thèses et écrits académiques. |
